National Maize Variety Test DNA Fingerprinting Management Measures (Trial)

Article 1 In order to strengthen the test management of maize varieties and improve the consistency and authenticity of the national varieties of tested maize varieties, the present Measures are formulated in accordance with the “Approval Methods for Major Crop Varieties”.
Article 2 The DNA fingerprint identification referred to in this method is based on the determination of the consistency and authenticity of the varieties based on the DNA fingerprinting bands of different species. The results of the test are used as the basis for the elimination of the test species, continued testing, and recommended validation.
Article 3 This method is based on the National Standard for Testing the Consistency and Authenticity of DNA Testing of Maize Varieties.
Article 4 According to the requirements of the annual test plan for submitting samples, the test host or unit responsible for numbering shall provide the seed samples required for DNA fingerprinting to the designated testing units in a timely, sufficient, and quality-reliable manner.
Article 5 The backup samples of regional trials and preliminary trials shall be kept for more than 3 years, and shall be preserved for more than 10 years by the national backup samples of the certified species, and shall be used for the species test or the standard sample for inspection and identification.
Article 6 According to the performance of the field variety traits, the degree of consistency between the preliminary test and the first year regional test variety shall be checked; and the consistency degree of the regional test variety entering the second year shall be checked.
Article 7 The consistency check is a grade 1 to 3, which can be tested normally or recommended for review. For a consistency test that is a grade 4 to 5, the test is stopped or not recommended for review.
Article 8 Both the preliminary test and the regional test shall be tested for authenticity, and the degree of difference between the test species and the known species shall be examined; the degree of difference between the same test species during interannual and different groups shall be determined.
The standard DNA fingerprinting of this species was synchronized.
Article 9 The same type of cessation test was found in different experimental years, different experimental groups, national and provincial trials with large genetic differences (differential sites ≥ 2).
It was found that the genetic divergence between the test cultivar and the known cultivar was small, that is, the test was stopped with the same or high degree of similarity (differential sites ≤ 1).
Article 10 Two (or more than two) test cultivars with different names are genetically identical or highly similar (differential loci ≤ 1) and are negotiated into unanimous written opinions by the participating units and submitted to the National Crop Variety Approval Committee for Maize. Professional committee, determine handling opinions.

Article 11 For varieties that have not passed the consistency or authenticity test, the provincial competent seed management department shall be informed by the competent department of the trial, and the provincial seed management department shall promptly notify the participating units of the variety to the test results.
Article 12 For the test units in different years, different groups, national and provincial tests during the test period, changing the variety of test units will stop the application qualification of all the varieties of this crop in the next year.
Article 13 Confirmation of adherence to the principle of prioritization of known species. That is, the application for the protection of new plant varieties, validation, and participation in national or provincial regional trials and production trials is prioritized. If national or provincial breed trials are to be carried out in the same order of protection or approval, the national trials will be given priority; if the participants participate in regional trials or production trials, the production trials will be given priority.

Article 14 The participating units of the varieties hold objections to the test results. Within 20 days after the information is obtained, please submit a written review to the Maize Professional Committee of the National Crop Variety Approval Committee. The Maize Professional Committee shall make reconsideration opinions within 60 days. The result is a final ruling.

Article 15 The present Measures shall be formulated by the Maize Professional Committee of the National Crop Variety Approval Committee;
Article 16 The present Measures shall be interpreted by the Maize Professional Committee of the National Crop Variety Approval Committee;
Article 17 These Measures shall be implemented as of the date of promulgation.

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