Comparison of several methods for determination of potato leaf area

Comparison of several methods for determination of potato leaf area

The leaf is the main site for photosynthesis of plants and is also the main route for transpiration. The size of the leaves has a significant impact on their own growth and development, light energy utilization, dry matter accumulation, yield and economic benefits. The determination of leaf area is often mentioned in botany, and is often studied. There are two types, traditional and modern. Traditional methods mainly work with rulers, but the accuracy is relatively low. Modern methods use relatively advanced technologies such as computers, scanners, or leaf area meters to measure the area of ​​the leaves and greatly increase the area of ​​the leaves. The accuracy of the leaf area is an important parameter for studying crop growth and development, population photosynthetic efficiency, and developing crop cultivation practices and technical standards.
Coefficient method: At the flowering stage, five consecutive plants were randomly selected in each plot. Two representative leaves were randomly selected from each plot. Ten leaves were measured in each plot, and a total of fifty leaves were determined.
Using the paper-cut weighing method: determine the actual area of ​​each tested blade S (ii = 1 ~ 50), the sum of the actual area of ​​the 50 blades Sa; At the same time record the maximum length of each measured blade L (i from the leaf Base to tip, not including petiole) and maximum width N (the widest point in the direction perpendicular to the main vein on the i leaf), Li×Ni is the approximate leaf area Si′, the sum of the approximate area of ​​the 50 leaves is denoted by Sb, The correction factor k is obtained with Sa/Sb.
Determination of the standard paper weight per unit area: Select 50 pieces of thick and thin uniform rectangular paper (this test uses copper plate paper, the area must be greater than the maximum value of the potato to be measured), measure its length and width, find the area S, and then The electronic balance (AR1140/C, 0.000 1 g) was weighed to obtain the weight W and the standard area weight per unit area = W/S.
Determination of the actual area Si: The paper is tightly attached to the lower part of the measured living body blade, and the shape of the tested blade is accurately traced on the paper along the outline of the leaf, including the small lobes on the potato leaf. Then use a pair of scissors to cut the paper leaves along the outline of the leaf to obtain a leaf paper mold. Weigh the paper mold weight Wi on an electronic analytical balance and divide the weight by the standard paper weight per unit area (W/S). The actual area of ​​the blade is Si.
Instrumental method: The leaf area is determined using a live leaf area meter or a portable leaf area meter. The leaf area meter for living leaves has a relatively large range of leaf sizes and can measure any size of leaf area with high accuracy. However, it has a disadvantage in that the leaves must be removed. It will cause damage to the plant, and the portable leaf area meter does not need to take off the leaf and can be measured directly on the leaf.

The above detailed analysis of several methods to determine potato leaf area, these methods need to be considered in practice, and then choose the appropriate method. In addition, in botanical studies, we often use instruments such as handheld chlorophyll meters, which are also an integral part of plant physiology.

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